Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.245135 |
Chromosome: | chromosome_2 |
Location: | 5612797 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g109150 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCTCAGAGCTAGAAGAAGTCTATTGTCTC |
Internal bar code: | CTCTAGATCTCCCCGCGCGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 44 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 88 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGGTGATATCTGCTGGGG |
Suggested primer 2: | GACTGCTGCGTCAACCTGTA |