Insertion junction: LMJ.RY0402.245155_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.245155
Chromosome:chromosome 3
Location:8291491
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g199200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGAAGCTGGAGTTACGCGCGCGGCACA
Internal bar code:ATACACGATAAGTCGGAGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:166
LEAP-Seq percent confirming:96.4497
LEAP-Seq n confirming:163
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATCGTTAGGCTCAGGTA
Suggested primer 2:ACACCCACACATACGCAAGA