| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245172 |
| Chromosome: | chromosome 12 |
| Location: | 7829562 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553250 | PFK2 | (1 of 3) K00850 - 6-phosphofructokinase 1 (pfkA, PFK); Phosphofructokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACACACACCCGGCTCCGCCGCTGCCC |
| Internal bar code: | CATGACCGGAAGACGCGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGTGTAACGGCACATCA |
| Suggested primer 2: | GGTTGGCAGTTCTCTCTTGC |