| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245196 |
| Chromosome: | chromosome 12 |
| Location: | 8583160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547350 | CYG31 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTTCCTGGTGGGTGCCAGCGCGTGGCG |
| Internal bar code: | ACCGTAGGGAGGGCCGCAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 94.7908 |
| LEAP-Seq n confirming: | 6569 |
| LEAP-Seq n nonconfirming: | 361 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCTTAGAGCAGCTCCC |
| Suggested primer 2: | ACGATGATGGGACTTTTTCG |