| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245199 |
| Chromosome: | chromosome 16 |
| Location: | 1230407 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650950 | (1 of 1) PF01931 - Protein of unknown function DUF84 (NTPase_I-T) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGATAGCAGAGAGTTGACCTGACCCCAC |
| Internal bar code: | ATTCGATGGACTCTTGCGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1314 |
| LEAP-Seq percent confirming: | 99.4203 |
| LEAP-Seq n confirming: | 19550 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAGCAAACCTGCAACAC |
| Suggested primer 2: | TCAATTACATCTCAGGGGGC |