| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245206 |
| Chromosome: | scaffold 35 |
| Location: | 13316 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre35.g759297 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACATGTAAGGTGAGTCAAACAGCAGCT |
| Internal bar code: | GTAGGATTTTGACCGTTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 98.5465 |
| LEAP-Seq n confirming: | 1017 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACAAACACTCGACCAATG |
| Suggested primer 2: | CCGAGGTAAGGCAAATGTGT |