| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245213 |
| Chromosome: | chromosome 1 |
| Location: | 4194685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027764 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCGCGCCAAAGCCGGGGCCAGGCAGT |
| Internal bar code: | TCAGGCGAAACAACCCTCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 98.7909 |
| LEAP-Seq n confirming: | 8252 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATGCGGAAGGTTTGTA |
| Suggested primer 2: | CTACCCTGCAGCATTTGGAT |