| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245225 |
| Chromosome: | chromosome_7 |
| Location: | 2058056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g325762 | DRC11 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGGTGTTCGGGGATGGTGGATGCCGGTG |
| Internal bar code: | TTGTTGTTGCGGGCTTACTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.4177 |
| LEAP-Seq n confirming: | 3927 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAAAGCTCTCCCGAAGT |
| Suggested primer 2: | AAGTTCATGGACAAAACGCC |