Insertion junction: LMJ.RY0402.245228_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.245228
Chromosome:chromosome 16
Location:1756339
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGACACGCCACGGCGACGACTCACCCC
Internal bar code:GTAGCAAGCGAAATGTAATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:501
LEAP-Seq percent confirming:97.6177
LEAP-Seq n confirming:1680
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGGTCATCTCGGATCTGT
Suggested primer 2:ACACACACACACACACACGC