| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245256 |
| Chromosome: | chromosome 5 |
| Location: | 2528686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAGGAGACCCGCCACCACCGGGCACAT |
| Internal bar code: | ACTCAGCACGAGGGTATTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 2706 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGGACGTCCATTCATAC |
| Suggested primer 2: | GACCTGTACATGGGCGTGTT |