Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.245294 |
Chromosome: | chromosome_6 |
Location: | 5919666 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g288550 | ECP76 | 76 kDa extracellular polypeptide | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCATCAAGACAGCCAGGGTTGTCCGCCCAT |
Internal bar code: | GGGACGGCAGTGGTCAGTCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1010 |
LEAP-Seq percent confirming: | 99.5038 |
LEAP-Seq n confirming: | 8222 |
LEAP-Seq n nonconfirming: | 41 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCGACTACACCATCACCTT |
Suggested primer 2: | CCAAAGTTCCCAGTTAGGCA |