| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245309 |
| Chromosome: | chromosome 17 |
| Location: | 5446676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737050 | (1 of 1) PTHR22957//PTHR22957:SF287 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCCGGCAGCCGCCGAGACAGAGTGTCG |
| Internal bar code: | TCCTGGCCTGATTGCGGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 765 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGATGAGGGGTGTTACG |
| Suggested primer 2: | GCTCCCACACAACCCTCTAA |