| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245311 |
| Chromosome: | chromosome 15 |
| Location: | 496185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636750 | GST11 | (1 of 15) 2.5.1.18 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase; Glutathione S-transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATAGAGTTTGAGCAAGGCTAGCTCAA |
| Internal bar code: | GGTTTTACTACTGCCCAGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 91.358 |
| LEAP-Seq n confirming: | 222 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTACGGGTTCCTGCTGT |
| Suggested primer 2: | AGTCCTCCTGGGTTTGTGTG |