Insertion junction: LMJ.RY0402.245340_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.245340
Chromosome:chromosome 9
Location:4110572
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g393654 RLS11 RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGGTCGGGCGGTCCACGGGCTTGCTCA
Internal bar code:GCGTAGGTGATTGGATGCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:96.4083
LEAP-Seq n confirming:1020
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAGCTAGGTTTCTTCGG
Suggested primer 2:TCTTGAATCATGCGTTGAGC