Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.245357 |
Chromosome: | chromosome_3 |
Location: | 5003191 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g180450 | FAP215,FPN1 | 5\'-nucleotidase and Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTACTGAGGTGCGGATGTCATCAACATGTG |
Internal bar code: | AGGCCCAGAAGCTAGAGACTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 34 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 233 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAAGCACAACGGCAGATA |
Suggested primer 2: | CTTTTGGGGCAGCATGTACT |