Insertion junction: LMJ.RY0402.245357_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.245357
Chromosome:chromosome_3
Location:5003282
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g180450 FAP215,FPN1 5\'-nucleotidase and Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GAAACGTTCAGTGCCCACCGTCCTAGTAAA
Internal bar code:CACCGATCAACGGGAATTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:96.7308
LEAP-Seq n confirming:503
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAAGCACAACGGCAGATA
Suggested primer 2:CCATAGGACTTCTTTGGGCA