Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.245357 |
Chromosome: | chromosome_3 |
Location: | 5003282 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g180450 | FAP215,FPN1 | 5\'-nucleotidase and Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAACGTTCAGTGCCCACCGTCCTAGTAAA |
Internal bar code: | CACCGATCAACGGGAATTCTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 640 |
LEAP-Seq percent confirming: | 96.7308 |
LEAP-Seq n confirming: | 503 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAAGCACAACGGCAGATA |
Suggested primer 2: | CCATAGGACTTCTTTGGGCA |