| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245366 |
| Chromosome: | chromosome 6 |
| Location: | 2518779 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAACAGTGGCACGCGCCATCCGAGCCAA |
| Internal bar code: | ACGCAAAGCCGGCCGGAATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 98.7724 |
| LEAP-Seq n confirming: | 1931 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACTGCCTGGGTATGAG |
| Suggested primer 2: | AGCAAGCAGGAGTGTACCGT |