| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245373 |
| Chromosome: | chromosome 3 |
| Location: | 7244549 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207600 | NSG6 | expressed during nitrogen-starved gametogenesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTATATGGCGCAAAATTGCGCCTCCTCC |
| Internal bar code: | TCCTTTGGCCCCCCCTCCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 98.3651 |
| LEAP-Seq n confirming: | 1083 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCAGTGTTCTGCTACAT |
| Suggested primer 2: | CTTTACTTCTGCGTCGGAGG |