Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.245374 |
Chromosome: | chromosome_17 |
Location: | 2400289 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g715300 | PKD2 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGAAGACCAAGGGCAACTTCCTCAAGTAC |
Internal bar code: | CGGCAGACATCCATCAGGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 579 |
LEAP-Seq percent confirming: | 99.4764 |
LEAP-Seq n confirming: | 1330 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTGGTTCACCTACCTCCAA |
Suggested primer 2: | GCAACATGCGCGTAAGTAAA |