| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245382 |
| Chromosome: | chromosome 7 |
| Location: | 564439 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316526 | (1 of 8) 3.1.1.13//3.1.1.3 - Sterol esterase / Triterpenol esterase // Triacylglycerol lipase / Triglyceride lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGCCTTAGAGCCGGTGTGTTTCTGGC |
| Internal bar code: | CCTCATCGTGTTAAAGCTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 96.9925 |
| LEAP-Seq n confirming: | 258 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACCTTACCTCCACGCCAC |
| Suggested primer 2: | CTAGGCCCTCTAGTACGCCA |