Insertion junction: LMJ.RY0402.245393_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.245393
Chromosome:chromosome 6
Location:1751244
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g262000 SNR5 SNARE-associated Golgi protein; (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGATGGTCATTTCTAACGGGGCACAGGCG
Internal bar code:ATTTAATTGTGGAACGGTCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:719
LEAP-Seq percent confirming:99.5278
LEAP-Seq n confirming:1897
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTAATGCCGAGTTGCTG
Suggested primer 2:TGTAGACGAGTCGCAGAACG