| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245404 |
| Chromosome: | chromosome 12 |
| Location: | 2111161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509150 | (1 of 1) PTHR18937//PTHR18937:SF151 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCCTGCACTTGCCTGGTTCCTCCCGTG |
| Internal bar code: | GGGCTTCCCTCGGACCACGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 95.9444 |
| LEAP-Seq n confirming: | 4211 |
| LEAP-Seq n nonconfirming: | 178 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTCACACAGGCGAAGAG |
| Suggested primer 2: | AAGGTCTCCGGATCCAGTCT |