| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245433 |
| Chromosome: | chromosome 1 |
| Location: | 4594690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031500 | (1 of 1) 1.10.3.2//1.10.3.3 - Laccase / Urishiol oxidase // L-ascorbate oxidase / Ascorbase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAGCCCTGCACTGAACTTGACCCCCCG |
| Internal bar code: | GGCCAAAATCGGGCCAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGTACTTGGGGTCCTTG |
| Suggested primer 2: | TATGGTAAGCATCAGCTGCG |