| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245452 |
| Chromosome: | chromosome 3 |
| Location: | 4845431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179050 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTAGTGCTGCTTATTTCTCTCTGCCAGA |
| Internal bar code: | GGTTCTGGTGATGGTTATTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 96.989 |
| LEAP-Seq n confirming: | 6861 |
| LEAP-Seq n nonconfirming: | 213 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTGGCATGCATTATGT |
| Suggested primer 2: | CATTGTCAACGTCATCGTCC |