| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245493 |
| Chromosome: | chromosome 6 |
| Location: | 4359804 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278267 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCAGCACAGGCCCCGCAACCCTACGTGC |
| Internal bar code: | GCTCTCTATCACTTTATTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.299 |
| LEAP-Seq n confirming: | 3683 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCACCGCATTACCTACT |
| Suggested primer 2: | ATCACCGTAACGCATGAACA |