| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245521 |
| Chromosome: | chromosome 16 |
| Location: | 6003054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676500 | (1 of 1) PTHR17630//PTHR17630:SF54 - DIENELACTONE HYDROLASE // ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGATGCTGGTGGTGGAGGTGGGCGTG |
| Internal bar code: | TACCCGCAACCAGCGGTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 90.5137 |
| LEAP-Seq n confirming: | 2872 |
| LEAP-Seq n nonconfirming: | 301 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGGAGACTCTGGATGC |
| Suggested primer 2: | CAACAACATGACGTCCAAGG |