| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245530 |
| Chromosome: | chromosome 16 |
| Location: | 1381464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652050 | ZXE3 | (1 of 1) PTHR13789//PTHR13789:SF234 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAATGCCGGAGTTAGACATGCCTGCAG |
| Internal bar code: | TGTAATCTCTAACTTTTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.5861 |
| LEAP-Seq n confirming: | 4571 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGGGCAGGTTGTGTAG |
| Suggested primer 2: | ACGACAAAACCAAAACCAGC |