| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245533 |
| Chromosome: | chromosome_1 |
| Location: | 4325321 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029250 | AAH1 | Aromatic amino acid hydroxylase-related protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTGGTGTTGTCCCCCGCCCCTGTTGTGA |
| Internal bar code: | TGTATGAGTAGAATAACATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 98.3225 |
| LEAP-Seq n confirming: | 1055 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACATCAGGAGTCAGCAA |
| Suggested primer 2: | CTTTGAAGAACGTTCGGAGC |