Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.245558 |
Chromosome: | chromosome 7 |
Location: | 4669677 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g344600 | PGD1,PGDH1 | (1 of 1) PTHR10996:SF159 - D-3-PHOSPHOGLYCERATE DEHYDROGENASE; D-3-phosphoglycerate dehydrogenase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCCCCCGCCGCTCGGCAGGGCGTGCT |
Internal bar code: | AGGGACCAGGTGATAGGGGACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 211 |
LEAP-Seq percent confirming: | 96.9697 |
LEAP-Seq n confirming: | 64 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
Suggested primer 2: | CCCTGGTTGTGGTTCAGTCT |