Insertion junction: LMJ.RY0402.245565_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.245565
Chromosome:chromosome 17
Location:418542
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g698903 (1 of 1) IPR000014//IPR002395 - PAS domain // HMW kininogen CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCGATGCCGTACGACACAAACGTGAG
Internal bar code:TGGCACTATAGGATATTTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:519
LEAP-Seq percent confirming:89.8277
LEAP-Seq n confirming:1616
LEAP-Seq n nonconfirming:183
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGTAAGTAACTCCGTCGC
Suggested primer 2:ACACACACACACACACACGC