| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245581 |
| Chromosome: | chromosome 12 |
| Location: | 9667497 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554953 | ETF2,ETFB1 | Electron transfer flavoprotein beta subunit; (1 of 1) K03521 - electron transfer flavoprotein beta subunit (fixA, etfB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGCTGTAGCAGGGAGGAGGGTTTGGG |
| Internal bar code: | TAGGACGGAGCGTATCGCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACCACG |
| Suggested primer 2: | TCAGCAGCAACTTGGACAAC |