| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245583 |
| Chromosome: | chromosome 6 |
| Location: | 8025055 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304150 | (1 of 4) PF06985//PF12796 - Heterokaryon incompatibility protein (HET) (HET) // Ankyrin repeats (3 copies) (Ank_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGGTGGAAAGCAAGCAGGTAAAGGATT |
| Internal bar code: | AATGCAAACTGTGACTAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.3345 |
| LEAP-Seq n confirming: | 7314 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAACGCACGTATTCAT |
| Suggested primer 2: | CATGCAGTGTGCTGTTGATG |