| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245583 |
| Chromosome: | scaffold 18 |
| Location: | 202100 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749597 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTTTCCCGGAGATGATAAGTTCCTCG |
| Internal bar code: | ATTTTTTTTAGAGTCTTATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 99.6156 |
| LEAP-Seq n confirming: | 6478 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCACTTGCTGTCGGCTT |
| Suggested primer 2: | CACAAGGCTGGTTGGATCAC |