| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245642 |
| Chromosome: | chromosome 3 |
| Location: | 5437592 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185150 | (1 of 11) IPR023109 - Integrase/recombinase, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGGGCAAAAACAGCCGACGTCGCCAG |
| Internal bar code: | CTCCCCGGGCCACGAGGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 82.0742 |
| LEAP-Seq n confirming: | 1195 |
| LEAP-Seq n nonconfirming: | 261 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCACGGCTATTTGACGTT |
| Suggested primer 2: | TAGCGATTGAGCGATACACG |