| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245645 |
| Chromosome: | chromosome 14 |
| Location: | 1498556 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618400 | ABCA3 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTGATACCATGAGTGGAACGCTTGTGA |
| Internal bar code: | GACTGTTCATATGGGCCTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 98.6543 |
| LEAP-Seq n confirming: | 4252 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAGGTAGAGAGGTGCG |
| Suggested primer 2: | CGCATGACAGGATAAGCAGA |