Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.245674 |
Chromosome: | chromosome_1 |
Location: | 3392842 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g021550 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACACACAGTAACCCGACCCGGTTGGGTGC |
Internal bar code: | GGGTTGCAACATTCCGGTTTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 548 |
LEAP-Seq percent confirming: | 99.8497 |
LEAP-Seq n confirming: | 1329 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCTGGGAGTCAAGGTCTG |
Suggested primer 2: | TGAGTCAGTGACAGGCAAGG |