| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245675 |
| Chromosome: | chromosome 9 |
| Location: | 4182662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394065 | FAP414 | (1 of 2) K18635 - protein SPIRAL1 and related proteins (SPR1); Flagellar Associated Protein 414 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCACTTCGGCTAAGCGCGTCACATGGTG |
| Internal bar code: | CGTGCGCTGGTTTTCTTGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 627 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCTACCTCGCGACTTC |
| Suggested primer 2: | GGAAACTACGCGTTGGGTTA |