| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245710 |
| Chromosome: | chromosome_2 |
| Location: | 8402838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141606 | DHC9 | Flagellar inner arm dynein heavy chain, dynein c (monomeric) | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGGTGGGCGCATGCGCGTGTATTTATGTG |
| Internal bar code: | TCTAGGCTGCAGTTTATATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 85.7351 |
| LEAP-Seq n confirming: | 589 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCTTGAACGTCTGCTT |
| Suggested primer 2: | TACCGCTTTTCCAAACATCC |