| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245725 |
| Chromosome: | chromosome 17 |
| Location: | 5831747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739650 | MAE1,MFT1 | MATE efflux family protein; (1 of 5) PTHR11206//PTHR11206:SF94 - MULTIDRUG RESISTANCE PROTEIN // DNA-DAMAGE-INDUCIBLE PROTEIN F | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTAATGACGTAATGTAAGGGGTCCTGC |
| Internal bar code: | CACTCGTTCCCCTGTGCTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 98.4503 |
| LEAP-Seq n confirming: | 1080 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAAGCAGTCAATGGGT |
| Suggested primer 2: | AGATTGCTTGCTTTCGCAAT |