| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245735 |
| Chromosome: | chromosome 7 |
| Location: | 4979341 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346800 | FAO8 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF204 - FAD NAD BINDING OXIDOREDUCTASES // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAAGCAGTGGTGGTGGTGGTGGTGGTG |
| Internal bar code: | TCTGTTATAGTAGATCGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 99.7347 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCATGATGGAAATGAG |
| Suggested primer 2: | ACACTAATCGGGACTGCGAC |