Insertion junction: LMJ.RY0402.245740_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.245740
Chromosome:chromosome 9
Location:6567944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g408100 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTGCGGCGTGATGAGCGGGTGGTAGGGG
Internal bar code:CACCGGGCTGATCGGCCGTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:666
LEAP-Seq percent confirming:19.7505
LEAP-Seq n confirming:855
LEAP-Seq n nonconfirming:3474
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGTGTGAGGTGCGGTAT
Suggested primer 2:CCAAACTAACCCAAGCCAAA