| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245754 |
| Chromosome: | chromosome 16 |
| Location: | 3111361 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665550 | (1 of 1) PTHR15492//PTHR15492:SF1 - FAMILY NOT NAMED // CYCLIN-D1-BINDING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGTGCACGGAATGGGCTGGTCAGGGT |
| Internal bar code: | TCGAGGAGTCGCCGAATTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 99.5322 |
| LEAP-Seq n confirming: | 2553 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAGTTCGGTATCATCGC |
| Suggested primer 2: | ACGGTAATCGTTGAACTGGC |