| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245756 |
| Chromosome: | chromosome 9 |
| Location: | 3010714 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387450 | OPR37 | OctotricoPeptide Repeat protein 37 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCGGGCGTGCGGCAGGGCAGTCCTTC |
| Internal bar code: | ACGGTCTTGATCGGCCCTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 20 |
| LEAP-Seq percent confirming: | 97.4952 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCCCCTACTTAGGTTC |
| Suggested primer 2: | CGAGAAGCGGAAAAATTGAG |