| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245769 |
| Chromosome: | chromosome 1 |
| Location: | 5333668 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037400 | CYN3,CYN19A,CYN19-1 | (1 of 2) K12868 - pre-mRNA-splicing factor SYF2 (SYF2); Cyclophilin 19-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGTCAGCCGCCTTGGCCCTCTCATAG |
| Internal bar code: | TCTGGGATAGTTCGACTTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 44.3223 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCGGCTCTTAAGTATG |
| Suggested primer 2: | TCAGGCATCAAAGCTGACAC |