| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.245810 |
| Chromosome: | chromosome 4 |
| Location: | 1740228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212350 | (1 of 2) PF10013 - Uncharacterized protein conserved in bacteria (DUF2256) (DUF2256) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTATGGGTAGTTTAAGAGCCGGAAGCGT |
| Internal bar code: | TCTGTAACGGATTGTGCTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.3694 |
| LEAP-Seq n confirming: | 1103 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCAGTGATTTCTGCTC |
| Suggested primer 2: | GTGCTTACTGCCAAGCATGA |