Insertion junction: LMJ.RY0402.245818_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.245818
Chromosome:chromosome 3
Location:9089705
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g211073 (1 of 2) K14497 - protein phosphatase 2C (PP2C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATCACCAGCGCTTGGCGTGTGAGCACC
Internal bar code:ATCATGAACAACCTGACGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:99.5112
LEAP-Seq n confirming:1018
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGGTCGTATGGTAAGGTG
Suggested primer 2:CCAGGTCAAGAGACCTCAGC