| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245820 |
| Chromosome: | chromosome 17 |
| Location: | 3424480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724400 | FAP257 | Flagellar Associated Protein 257; (1 of 3) PF05217 - STOP protein (STOP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTTGGAGGCCCATGCAGGCCCATGCGT |
| Internal bar code: | CCGGCTTGGCATCTCAAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 99.4553 |
| LEAP-Seq n confirming: | 3834 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACCATAAACGCAACAT |
| Suggested primer 2: | CACGACCATGATTAACGTGC |