Insertion junction: LMJ.RY0402.245862_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.245862
Chromosome:chromosome 6
Location:5457067
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g284400 (1 of 1) K06695 - 26S proteasome regulatory subunit, ATPase 3, interacting protein (PSMC3IP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCGGATCCCCACCACCAGTTCTTAGGCA
Internal bar code:GGGGGTGATAGAAGGCGAAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:248
LEAP-Seq percent confirming:99.4495
LEAP-Seq n confirming:1084
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGGACAGATGACTGCAA
Suggested primer 2:CGTGTGGAACATGAAAGGTG