| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245875 |
| Chromosome: | chromosome 8 |
| Location: | 2530951 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372250 | YDIF1,YDIF | Putative soluble chloroplast ABCF protein, related to bacterial YdiF.; (1 of 1) PTHR19211//PTHR19211:SF48 - ATP-BINDING TRANSPORT PROTEIN-RELATED // ABC TRANSPORTER F FAMILY MEMBER 2-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCGGCACTGAGCGAGCCCAACGAGTTC |
| Internal bar code: | TGTGGGCATGAAGCGGGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 98.374 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTTTACTGAGGGCTGTT |
| Suggested primer 2: | GCGTTACGACTTGGAATGGT |