| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245921 |
| Chromosome: | chromosome 14 |
| Location: | 2344058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623800 | NAT6 | N-acetyltransferase; (1 of 1) PTHR23091//PTHR23091:SF66 - N-TERMINAL ACETYLTRANSFERASE // N-ALPHA-ACETYLTRANSFERASE 30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATGGATTTCTCAGTGATCCACTGCCAG |
| Internal bar code: | TACGTTTACCTAGTTGCTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 94.9772 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAAGGCTGGAGGTGGTG |
| Suggested primer 2: | GTTGTTCAGGAGGTGGCACT |